Journal: CNS Neuroscience & Therapeutics
Article Title: Aloe‐Emodin Improves Mitophagy in Alzheimer's Disease via Activating the AMPK / PGC ‐1α/ SIRT3 Signaling Pathway
doi: 10.1111/cns.70346
Figure Lengend Snippet: AE treatment modulates mitophagy and proteins related to the AMPK/PGC‐1α/SIRT3 pathway. (A) Western blot results of AMPK, p‐AMPK, PGC‐1α, SIRT3, and mitophagy‐related proteins (LC3B, P62, Beclin1) in HT22 cells. (B) Statistical analysis of the western blotting results. (C) Fluorescence staining of p‐AMPK, AMPK (red), and DAPI (blue) among HT22 cells. (D) Fluorescence staining of LC3B (green) and DAPI (blue) among the ditto cells. (E) Fluorescence staining of P62 (red) and DAPI (blue) among the ditto cells. Scale bar = 20 μm. (F) Statistical analysis on the IF results. The outcomes were denoted as mean ± SEM( n = 3). # p < 0.05 by contrast with the WT group, * p < 0.05 by contrast with the ad group.
Article Snippet: Diluted primary antibodies LC3B (Zenbio), Parkin (Zenbio), SIRT3 (Affinity), and p‐AMPK (Beyotime) were added and nurtured at 4°C.
Techniques: Western Blot, Fluorescence, Staining